Fig. 4

VCP inhibition results in G1 cell cycle arrest.a Propidium iodide incorporation/FACS analysis of vehicle-treated (white columns) or EER-1-treated (black columns) cells. Cells were gated and counted as described below and in the Methods section and grouped into the G0/G1 or in S/G2/M phases. Data are presented as mean (columns) ± SEM (error bars), n = 3 analyses/treatment. Asterisks indicate a statistically significant difference (P < 0.001) from vehicle-treated group. The experiment was repeated three times and a representative result is shown. b Representative two parameter dot-plot (FL2-A (area) vs width); the gray rectangle represents the population of cells analyzed in the FACS experiment. Representative FACS analyses of vehicle-treated (c) and EER-1-treated (d) cells. Gray arrows indicate the populations that were defined as G0/G1, S and G2/M