Fig. 3

VCP inhibition induces apotosis in canine lymphoma cell lines. a Representative images of TUNEL assays done on CLBL-1 cells following treatment with vehicle (left panel), 1 μM (middle panel) and 3 μM (right panel) of EER-1 treatment. Live cells appear blue (DAPI signal) and an apoptotic cells appear purple (merged red TUNEL signal + DAPI). b Quantitative analysis of the TUNEL assays . c Caspase 3/7 activity assay. Data are presented as means (columns) ± SEM (error bars). Asterisks indicate a statistically significant difference (*P < 0.05; **P < 0.01 or ***P < 0.001) compared to the vehicle. For both experiments, n = 3 replicates per treatment, each experiment was repeated three times and a representative result is shown