Fig. 2

VCP inhibition decreases canine lymphoma cell viability. PBMCs, CLBL-1, 17–71 and CL-1 cells were treated for 48 h with graded doses of EER-1. Cell viability was evaluated by trypan blue exclusion. At 1 and 2 μM doses, the proportion of viable PBMCs was significantly higher than in CL-1 cells (p < 0.05 and p < 0.01, respectively). At 3, 4 and 5 μM, the proportion of viable PBMCs was significantly higher than CLBL-1, 17–71 or CL-1 cells (p < 0.001 for each cell line and for each inhibitor concentration). Data are presented as means ± SEM (error bars), n = 4 replicates per treatment. The experiment was repeated three times, and a representative result is shown