Fig. 1

VCP protein expression in canine lymphoma. a Immunoblotting analysis was done for VCP. Representative blots are shown, each lane represents a single lymph node or tumor sample from one patient. Quantitative analyses of were done using n = 4–9 tumor samples per grade and type and normalized to ACTB (β actin) as a loading control. Data are presented as mean (cross bar) ± SEM (error bars). Asterisk (*) indicates a statistically significant difference (*P < 0.05) compared to low grade. b VCP immunohistochemistry for B-cell lymphoma compared to normal lymph node. c Immunoblotting analysis of VCP in PBMCs (signal intensity = 1.01 arbitrary units) and lymphoma cell lines (17–71, CL-1 and CLBL-1) (average signal intensity = 3.33 arbitrary units). High, Inter (Intermediate) and Low refer to grade, T and B refer to lymphoma cellular subtype, LN = normal lymph node