Fig. 4

Immunofluorescent evidence of epithelial-mesenchymal trasition induced by constiutive Bcl-2 expression associated with the loss of epithelial markers and the gain of mesenchymal markers. Cells were fixed and immunofluorescent staining was performed using antibodies specific to E- and N-cadherin, β-catenin, desmoplakin I&II, vimentin and epithelial specific antigen (ESA) and FITC-conjugated secondary antibodies. The images were photographed under a Bio-Rad MRC1024 confocal scanning laser microscope (magnification X 60)