|
Cells
|
p53 status
|
Mutated codon
|
CAR expression
|
GFP-positive cells (%)
|
|---|
|
Ad5
|
AdF35
|
|---|
|
TE-1
|
mutated
|
Codon 272
|
6.57
|
8.06 ± 1.17
|
53.47 ± 0.10
|
|
TE-2
|
wild-type
| |
19.22
|
0.79 ± 0.14
|
10.15 ± 0.44
|
|
TE-10
|
mutated
|
Codon 242
|
14.73
|
16.15 ± 0.52
|
35.33 ± 0.67
|
|
TE-11
|
wild-type
| |
22.25
|
22.86 ± 0.53
|
42.82 ± 0.74
|
|
YES-2
|
mutated
|
Codon 236
|
0.19
|
5.09 ± 1.29
|
51.54 ± 0.36
|
|
YES-4
|
wild-type
| |
26.87
|
27.18 ± 0.16
|
61.23 ± 0.07
|
|
YES-5
|
mutated
|
Codon 280
|
15.40
|
22.18 ± 0.32
|
69.97 ± 0.89
|
|
YES-6
|
wild-type
| |
30.23
|
16.59 ± 0.25
|
27.63 ± 0.17
|
|
T.Tn
|
mutated
|
Codon 214 and 258
|
8.12
|
0.49 ± 1.00
|
21.60 ± 0.15
|
|
HEK293
| | |
56.97
|
87.20 ± 0.52
|
78.05 ± 0.70
|
- Cells were infected with Ad5/GFP or AdF35/GFP at a 30 MOI and were analyzed for the fluorescence with flow cytometry. Averages and the SEs are shown (n = 3). CAR expression levels were determined with flow cytometry and are expressed with an arbitrary unit
