Cytotoxic effects of replication-competent adenoviruses on human esophageal carcinoma are enhanced by forced p53 expression

Table 1 Infectivity of Ad5 and AdF35 to esophageal carcinoma cells and CAR expression levels

Cells	p53 status	Mutated codon	CAR expression	GFP-positive cells (%)
Cells	p53 status	Mutated codon	CAR expression	Ad5	AdF35
TE-1	mutated	Codon 272	6.57	8.06 ± 1.17	53.47 ± 0.10
TE-2	wild-type		19.22	0.79 ± 0.14	10.15 ± 0.44
TE-10	mutated	Codon 242	14.73	16.15 ± 0.52	35.33 ± 0.67
TE-11	wild-type		22.25	22.86 ± 0.53	42.82 ± 0.74
YES-2	mutated	Codon 236	0.19	5.09 ± 1.29	51.54 ± 0.36
YES-4	wild-type		26.87	27.18 ± 0.16	61.23 ± 0.07
YES-5	mutated	Codon 280	15.40	22.18 ± 0.32	69.97 ± 0.89
YES-6	wild-type		30.23	16.59 ± 0.25	27.63 ± 0.17
T.Tn	mutated	Codon 214 and 258	8.12	0.49 ± 1.00	21.60 ± 0.15
HEK293			56.97	87.20 ± 0.52	78.05 ± 0.70

Cells were infected with Ad5/GFP or AdF35/GFP at a 30 MOI and were analyzed for the fluorescence with flow cytometry. Averages and the SEs are shown (n = 3). CAR expression levels were determined with flow cytometry and are expressed with an arbitrary unit

ISSN: 1471-2407