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Fig. 5 | BMC Cancer

Fig. 5

From: Cytotoxic effects of replication-competent adenoviruses on human esophageal carcinoma are enhanced by forced p53 expression

Fig. 5

Production of anti-tumor effects with replication-competent Ad and Ad5/p53. a Representative flow cytometrical analyses about cell cycle changes. YES-2 cells were uninfected or infected with AdF35/MK (1.2 × 103 vp/cell), Ad5/p53, Ad5/LacZ (6.5 × 103 vp/cell) or in combination, and were analyzed on day 2. b Anti-tumor effects in vivo produced by AdF35/MK and Ad5/p53. Nude mice that were subcutaneously injected with YES-2 cells (1x106) were treated with Ad as indicated (1.875x108 pfu/mouse) on day 0, 3, 6 and 9 after the tumors developed to 65 mm3 (n = 5). The tumor sizes were measured every 4 days. Means and SE bars are shown. *P < 0.01. c Influence of Ad/p53 on viral proliferation of replication-competent AdF35. YES-2 cells were infected with AdF35/MK or AdF35/Sur, or in combination of AdF35/MK or AdF35/Sur and Ad5/p53 or Ad5/LacZ (AdF35/MK and AdF35/Sur: 1.2 × 103 vp/cell; Ad5/p53 and Ad5/LacZ: 6.5 × 103 vp/cell), and the cell lysate was extracted on day 3 or 4. The viral titers were assayed with TCID50 method with A549 cells. Means and SE bars are shown (n = 3). *P < 0.05. d Activation of the Ad5/p53-derived p53 pathways with AdF35/MK. YES-2 cells were uninfected or infected with AdF35/MK (1.2 × 103 vp/cell), Ad5/p53 or Ad5/LacZ (6.5 × 103 vp/cell) as a control, or in combination of AdF35/MK and Ad5/p53 or Ad5/LacZ for 2 or 3 days. Expression levels of molecules in the p53 pathways were analyzed with western blot analyses and those of α-tubulin were used as a loading control

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