Fig. 4
Quantification of radiosensitizing effect after Vinblastine treatment using T47D monotypic 3D-microtissues. GFP plot (area in μm2) for control T47D 3D-microtissue and after treatment with 0 Gy and 2 Gy irradiation using (a) 10 μM Vinblastine and (b) 300 nM Vinblastine at indicated time points. Data are averages ± SD; * indicate statistically (t-test) significant changes to corresponding control with Vinblastine treatment at 0 Gy, *p < 0.05; **p < 0.001. (c) T47D 3D-microtissues were treated with 0 Gy and 2 Gy radiation including 300 nM Docetaxel or 300 nM Vinblastine. 12 days after treatment 3D-microtissues were lysed directly in assay plates and measured for luciferase activity (Cell-TiterGlo Assay). Data are averages ± SD, * indicate statistically (t-test) significant changes to corresponding controls at 0 Gy, **p < 0.001