Fig. 1
Growth analysis of 3D-microtissues with constitutive lentiviral-GFP expression. a Example of Operetta bright-filed and GFP detection in breast cancer 3D-microtissue spheroids after indicated time points (days after seeding in assay plates). b 3D-microtissue growth analysis after radiation (GFP plot in μm2) – area quantification of spheroid growth delay after irradiation at indicated time points and different radiation doses. Data are averages ± SD (n = 3). c Example of Operetta bright-filed and GFP area detection 9 days after radiation treatment. Operetta laser scanning instrument operates with constant excitation times (1 ms), nevertheless fluorophore saturation was detected after radiation treatment, confirming that absolute fluorescence intensity cannot be used for quantification