Fig. 4

Effects of CAV1 down-regulation on ECC proliferation. Wild type, shLuc and shCav-1(#5) Ishikawa (a) and Hec-1A (b) cells were seeded in complete medium in 96-well plates 24 h prior to serum withdrawal for an additional 24 or 48 h of culture, respectively. After 24 h of 100 nM 4β-TPA treatment, cell proliferation was evaluated using the MTS® assay according to the manufacturer’s instructions. Data averaged from three independent experiments are shown (mean ± SEM). Statistically significant differences compared with the control group (shLuc cells without 4β-TPA, reference value 1 ) are indicated (*, p < 0.05)