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Fig. 3 | BMC Cancer

Fig. 3

From: Enhanced caveolin-1 expression increases migration, anchorage-independent growth and invasion of endometrial adenocarcinoma cells

Fig. 3

CAV1 expression increased in ECC after exposure to 4β-TPA. Ishikawa and Hec-1A cells were transduced with CAV1 shRNA (shRNA Cav-1-(#5)) or shRNA for Luciferase (shLuc), as a control. Stably transduced cells expressing the corresponding construct were obtained by selection in medium with puromycin. Wild type and transduced Ishikawa (a) and Hec-1A (b) cells were seeded in 6-cm dishes for 24 h in complete medium and then cultured in medium without serum for 24 h or 48 h, respectively, prior to 100 nM 4β-TPA stimulation for 24 h. CAV1 protein levels were determined by Western blot analysis. β-actin was used as internal control. Values obtained by scanning densitometric analysis of three independent experiments are shown for CAV1 following standardization to β-actin and normalization to shLuc cells without treatment (mean ± SEM). Data were analized using the unpaired t-test. Statistically significant differences compared with the controls are indicated (*, p < 0.05, **,p < 0.01). Note that levels detected for the shLuc cells without 4β-TPA were assigned the reference value 1

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