Fig. 2

Cytotoxic effects of MK1775 and AZD7762 are not reversed by medium renewal in 3D cultures. a. Experiment outline; spheroid formation was allowed prior to treatment (day 0–3), inhibitors (MK1775: 200nM, AZD7762: 100nM) were added on day 3. On day 6 and 10 50 % of the medium was substituted by fresh medium containing no inhibitors. b. Spheroids were visually assessed on day 14 using light microscopy; scales in bottom right corners show 500 μm. c. Spheroid viability was estimated by CellTiterGlow analysis on day 14, and presented as the average of four individual experiments. d. Spheroid volume (Y-axis) was measured/estimated using Olympus Soft Imaging Solution Gm6H software. Changes in volume were estimated for each spheroid relative to its size on day 3. The graphs represent the average of two individual experiments presented with positive standard deviations