Fig. 1

Increased anti-tumour effect of combined inhibition of Wee1 and Chk1/2. a and b, Melanoma cell lines grown as monolayer cultures were treated with increasing concentrations (0.063–1 μM) of MK1775 (a) or AZD7762 (b) for 48 h, and viability was measured by MTS analysis. c. Metastatic melanoma cell lines and primary cultures of human melanocytes (FOMA4) and fibroblasts (FF144 SC) were exposed to 200nM MK1775 and/or 100nM AZD7762 for 48 h. Cell viability was determined by MTS analysis. Data presented are the mean of at least three independent biological experiments and presented with positive standard deviation