Fig. 2

IL-10-stimulated macrophages are more efficient in stimulating cancer cell invasion. a AGS human gastric or b RKO human colorectal cancer cells were incubated in BD BioCoat^TM Matrigel^TM Invasion Chambers for 24 h with RPMI medium (-), or human macrophages differentiated for 10 days and stimulated for 72 h with 10 ng/ml LPS (LPSmac) or 10 ng/ml IL-10 (IL-10mac). Invasive cells and invasive ratio were determined as described in Materials and Methods. Bars represent mean values of independent experiments performed with, at least, 4 different donors; flags indicate standard deviations