Fig. 3
a Quantification of viable cells in Aza-reprogrammed (500 nM) Bz-surviving RPMI8226 cells compared to Bz only treatment, using Trypan blue exclusion assay. b & c IF detection and quantification of P-Rb (Ser249/Thr252) in RPMI8226 cells reprogrammed for 4 days ± 500 nM Aza, pulsed for 24 h with 4 nM Bz and stained 0, 3 (Additional file 3: Figure S2B) and 6 days after drug washout. d & e Detection and quantification of p21CIP1 in Aza-reprogrammed Bz-surviving RPMI8226 cells via IF. Scale bar =20 μm