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Fig. 3 | BMC Cancer

Fig. 3

From: miR-16 promotes the apoptosis of human cancer cells by targeting FEAT

Fig. 3

The role of miR-16 targeting FEAT in the regulation of apoptosis of cancer cells. a Quantitative RT-PCR analysis of FEAT mRNA levels in A549 cells treated with control siRNA, FEAT siRNA, control plasmid, and FEAT plasmid. b and c Western blot analysis of FEAT protein levels in A549 cells treated with control siRNA, FEAT siRNA, control plasmid, and FEAT plasmid. B: representative image; C: quantitative analysis. (D and E) A549 cells were transfected with equal doses of pre-mir-control, pre-mir-16, anti-mir-control, anti-mir-16, control siRNA, FEAT siRNA, control plasmid, FEAT plasmid, or with pre-mir-control plus control plasmid, pre-mir-16 plus control plasmid, pre-mir-control plus FEAT plasmid, or pre-mir-16 plus FEAT plasmid. Cell apoptosis profiles were analyzed by flow cytometry. The biparametric histogram shows cells in early (bottom right quadrant) and late apoptotic states (upper right quadrant). Viable cells are double negative (bottom left quadrant). d: representative image; e: quantitative analysis. ***, P < 0.001

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