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Fig. 2 | BMC Cancer

Fig. 2

From: miR-16 promotes the apoptosis of human cancer cells by targeting FEAT

Fig. 2

Direct regulation of FEAT expression by miR-16 at the posttranscriptional level. a Quantitative RT-PCR analysis of the miR-16 levels in A549, MCF-7, and Huh-7 cells transfected with pre-mir-control, pre-mir-16, anti-mir-control, and anti-mir-16. b and c Western blot analysis of the FEAT protein levels in A549, MCF-7, and Huh-7 cells transfected with pre-mir-control, pre-mir-16, anti-mir-control, and anti-mir-16. B: representative image; C: quantitative analysis. d Quantitative RT-PCR analysis of FEAT mRNA levels in A549, MCF-7, and Huh-7 cells transfected with pre-mir-control, pre-mir-16, anti-mir-control, and anti-mir-16. e Firefly luciferase reporters containing wild-type (WT) or mutant (MUT) miR-16 binding sites in the FEAT 3′-UTR were co-transfected into A549, MCF-7, and Huh-7 cells with pre-mir-control, pre-mir-16, anti-mir-control, and anti-mir-16. Twenty-four hours post-transfection, the cells were assayed using a luciferase assay kit. ***, P < 0.001

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