Fig. 1

Gene expression of ATP-dependent binding cassette transporters. a Microarray analysis of mammary glands from Apc^Min/+ and Apc^+/+ mice at d16 of lactation show a decrease in ABCG2 and increase in MDR1 expression due to Apc mutation. b MDR1 gene expression in cells from MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ mice after 24 h treatment with either solvent control, paclitaxel, cisplatin or doxorubicin. MDR1 expression was significantly increased in cells from MMTV-PyMT;Apc^Min/+ mice after treatment with paclitaxel and doxorubicin but not cisplatin. c ABCG2 gene expression in cells from MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ mice after treatment for 24 h with either solvent control, paclitaxel, cisplatin or doxorubicin. ABCG2 expression was not different between MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ cells and chemotherapy treatment had no effect on ABCG2 expression. d Representative western blots for MDR1 and ABCG2 in cells from MMTV-PyMT;Apc^Min/+ and MMTV-PyMT;Apc^+/+ mice after treatment for 24 h with either solvent control, paclitaxel, cisplatin or doxorubicin. e Quantification of MDR1 western blots shows that MMTV-PyMT;Apc^Min/+ cells have enhanced MDR1 expression when treated with doxorubicin. f Quantification of ABCG2 western blots shows that MMTV-PyMT;Apc^+/+ cells have elevated ABCG2 protein expression compared to MMTV-PyMT;Apc^Min/+ cells. Results in B, C, E and F are shown as the means ± SEM from 3 independent experiments; *P < 0.05 when comparing MMTV-PyMT;Apc^Min/+ to MMTV-PyMT;Apc^+/+ cells and #P < 0.05 when comparing MMTV-PyMT;Apc^Min/+ cells treated with solvent control or chemotherapy agent.