17β-estradiol induces stearoyl-CoA desaturase-1 expression in estrogen receptor-positive breast cancer cells

BMC Cancer

Table 1 Fatty acid composition of 17β-estradiol-treated and untreated cells

Fatty Acids	MCF-7		T47D		MCF-10A
Fatty Acids	Ctrl	17β-ED	Ctrl	17β-ED	Ctrl	17β-ED
16:0	19.0 ± 0.1	20.1 ± 0.4	29.6 ± 0.7	29.4 ± 1.5	20.6 ± 0.1	19.7 ± 1.4
16:1n-7	2.6 ± 0.2	9.0 ± 0.9^a	5.9 ± 0.8	8.1 ± 0.7^a	4.9 ± 0.1	4.4 ± 0.3
18:0	18.7 ± 0.9	13.1 ± 0.6^a	11.6 ± 1.0	9.1 ± 0.2^a	7.6 ± 0.1	7.5 ± 0.5
18:1n-9	22.0 ± 0.1	25.8 ± 0.4^a	27.6 ± 0.4	27.7 ± 1.2	38.4 ± 0.2	39.9 ± 2.7
18:1n-7	4.0 ± 0.05	7.2 ± 0.8^a	7.5 ± 0.5	9.7 ± 0.3^a	13.4 ± 0.0	13.5 ± 5.7
Ratios	Ctrl	E	Ctr	E	Ctrl	E
16:1n-7/16:0	0.1 ± 0.0	0.4 ± 0.05^a	0.2 ± 0.0	0.3 ± 0.0^a	0.2 ± 0.0	0.2 ± 0.0
18:1n-9/18:0	1.2 ± 0.1	2.0 ± 0.1^a	2.4 ± 0.2	3.1 ± 0.1^a	5.0 ± 0.0	5.3 ± 0.0
16:1n-7 + 18:1n-7/16:0	0.3 ± 0.0	0.8 ± 0.1^a	0.4 ± 0.0	0.6 ± 0.0^a	0.9 ± 0.1	0.9 ± 0.2

MCF-7, T47D, and MCF-10A cells were incubated for 7 days in phenol red-free media supplemented with charcoal-stripped FBS, followed by a 5 day incubation period in the same media that was supplemented with 2nM 17β-ED or its vehicle EtOH (Ctrl)
Cellular lipids were extracted and fatty acids methyl esters were prepared and measured. Values for each fatty acid represent the percentage of total cellular fatty acids. The results are the means ± SEM, n = 3 to 5 independent experiments. ^aDifferent from control (P < 0.05) as determined by student’s t-test.

ISSN: 1471-2407