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Table 1 Fatty acid composition of 17β-estradiol-treated and untreated cells

From: 17β-estradiol induces stearoyl-CoA desaturase-1 expression in estrogen receptor-positive breast cancer cells

Fatty Acids MCF-7 T47D MCF-10A
Ctrl 17β-ED Ctrl 17β-ED Ctrl 17β-ED
16:0 19.0 ± 0.1 20.1 ± 0.4 29.6 ± 0.7 29.4 ± 1.5 20.6 ± 0.1 19.7 ± 1.4
16:1n-7 2.6 ± 0.2 9.0 ± 0.9a 5.9 ± 0.8 8.1 ± 0.7a 4.9 ± 0.1 4.4 ± 0.3
18:0 18.7 ± 0.9 13.1 ± 0.6a 11.6 ± 1.0 9.1 ± 0.2a 7.6 ± 0.1 7.5 ± 0.5
18:1n-9 22.0 ± 0.1 25.8 ± 0.4a 27.6 ± 0.4 27.7 ± 1.2 38.4 ± 0.2 39.9 ± 2.7
18:1n-7 4.0 ± 0.05 7.2 ± 0.8a 7.5 ± 0.5 9.7 ± 0.3a 13.4 ± 0.0 13.5 ± 5.7
Ratios Ctrl E Ctr E Ctrl E
16:1n-7/16:0 0.1 ± 0.0 0.4 ± 0.05a 0.2 ± 0.0 0.3 ± 0.0a 0.2 ± 0.0 0.2 ± 0.0
18:1n-9/18:0 1.2 ± 0.1 2.0 ± 0.1a 2.4 ± 0.2 3.1 ± 0.1a 5.0 ± 0.0 5.3 ± 0.0
16:1n-7 + 18:1n-7/16:0 0.3 ± 0.0 0.8 ± 0.1a 0.4 ± 0.0 0.6 ± 0.0a 0.9 ± 0.1 0.9 ± 0.2
  1. MCF-7, T47D, and MCF-10A cells were incubated for 7 days in phenol red-free media supplemented with charcoal-stripped FBS, followed by a 5 day incubation period in the same media that was supplemented with 2nM 17β-ED or its vehicle EtOH (Ctrl)
  2. Cellular lipids were extracted and fatty acids methyl esters were prepared and measured. Values for each fatty acid represent the percentage of total cellular fatty acids. The results are the means ± SEM, n = 3 to 5 independent experiments. aDifferent from control (P < 0.05) as determined by student’s t-test.