Fig. 3

miR-17 regulates TRIM8 expression at the post-transcriptional level. a HEK293 cells were co-transfected with a reporter construct carrying the 3′ UTR of TRIM8 and a pcDNA3-miR-17-92 or pcDNA3 empty vector. Luciferase activities were measured and normalized to the level of control Renilla luciferase. b HEK293 cells were co-transfected with reporter constructs carrying the 3′UTR of TRIM8 or 3′ UTR of TRIM8 containing mutated miR-17 complementary site and a synthetic mimic of miR-17, mimic of miR-20a, or miR-control (miR-CNT). c Detection of TRIM8 endogenous expression by qPCR in MCF-7, HeLa, HEK293, and U87MG cell lines transfected with miR-17 mimic or miR-control. dTRIM8 endogenous expression in MCF-7 cell lines transfected with miR-17 inhibitor or miR-control inhibitor. e Immunoblotting analysis by using indicated antibodies on whole protein lysate from U87MG transfected with a construct carrying the ORF and 3′UTR of TRIM8 or the ORF and 3′ UTR of TRIM8 containing a deletion of miR-17 complementary site and a miR-17 mimic, miR-17 inhibitor, or miR-control