Table 1 RT-PCR primers sequences used for the amplification of multiple human cDNAs
Sl No | Gene | Annealing temperature (°C) | Product size (bp) | Sequence (5’ - 3’) |
|---|---|---|---|---|
1 | Cyclin-D1 | 58 | 135 | F – gctgcgaagtggaaaccatc |
R – cctccttctgcacacatttgaa | ||||
2 | IGF-1Rβ | 58 | 522 | F – actatgccggtgtctgtgtg |
R – tgcaagttctgattgttgag | ||||
3 | IGF-1 | 58 | 498 | F – tcctcgcatctcttctacct |
R – tctggactcgccagtccaat | ||||
4 | VEGF | 58 | 405 | F – aggagggcagaatcatcacg |
R – caaggcccacagggattttc | ||||
5 | 18S | 58 | 490 | F – agccttcggctgactggctgg |
R – ctgcccatcatcatgacctgg | ||||
6 | MMP2 | 53 | 665 | F – gagttggcagtgcaatacct |
R – gccatccttctcaaagttgt | ||||
7 | MMP3 | 60 | 432 | F – cctgctttgtcctttgatgc |
R – tgagtcaatccctggaaagt | ||||
8 | MMP9 | 58 | 455 | F – cctgccagtttccattcatc |
R – gccattcacgtcgtccttat | ||||
9 | hIGF-1 (CHIP assay) | 60 | 700 | F – tggcatgttttgaggttttg |
R – gattggttgtgtggcatgag |