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Table 1 Annexin-V results following bLf treatments in breast cancer cells

From: Iron-free and iron-saturated bovine lactoferrin inhibit survivin expression and differentially modulate apoptosis in breast cancer

   Early Apoptotic Cells Late Apoptotic Cells Viable Cells Necrotic Cells
MDA-MB-231 Untreated 1.75 ± 1.15 2.50 ± 2.10 94.85 ± 3.25 0.85 ± 0.05
Apo-bLf 20 nM 1.90 ± 0.60 1.10 ± 0.40 96.2 ± 1.40 0.85 ± 0.35
Apo-bLf 40 nM 5.55 ± 0.65 17.75 ± 0.05* 75.4 ± 0.10* 1.10 ± 0.5
Fe-bLf 20 nM 1.90 ± 0.30 9.30 ± 3.30 87.7 ± 3.40 1.10 ± 0.20
Fe-bLf 40 nM 3.75 ± 1.95 19.35 ± 6.85 74.00 ± 8.80 2.90 ± 0.00
MCF-7 Untreated 3.40 ± 3.20 5.25 ± 3.25 88.60 ± 7.30 2.70 ± 0.90
Apo-bLf 20 nM 2.50 ± 0.40 22.85 ± 9.65 64.95 ± 3.85 9.75 ± 6.15
Apo-bLf 40 nM 6.15 ± 0.55 40.7 ± 7.30* 51.00 ± 6.70* 2.20 ± 0.00
Fe-bLf 20 nM 12.30 ± 0.30 41.3 ± 1.40* 45.45 ± 1.65* 0.95 ± 0.05
Fe-bLf 40 nM 21.65 ± 1.65* 43.5 ± 12.10 34.15 ± 10.05* 0.70 ± 0.40
  1. Annexin-V-Fluos staining results determined via Flow cytometry. Total populations acquired were plotted in terms of both single and dual fluorophores. Dual channel plots were divided in to quadrants: Q1: Necrotic cells, Q2: Late apoptotic/Dead cells, Q3: Viable cells, Q4: Early apoptotic cells. Single channel plots were used to determine total numbers of cells labelled with Annexin-V or propidium iodide. Table 1 shows average values for individual gating of both dual and single channel plots + SEM. Statistical analysis was performed via Student’s t-test