Iron-free and iron-saturated bovine lactoferrin inhibit survivin expression and differentially modulate apoptosis in breast cancer

Table 1 Annexin-V results following bLf treatments in breast cancer cells

		Early Apoptotic Cells	Late Apoptotic Cells	Viable Cells	Necrotic Cells
MDA-MB-231	Untreated	1.75 ± 1.15	2.50 ± 2.10	94.85 ± 3.25	0.85 ± 0.05
	Apo-bLf 20 nM	1.90 ± 0.60	1.10 ± 0.40	96.2 ± 1.40	0.85 ± 0.35
	Apo-bLf 40 nM	5.55 ± 0.65	17.75 ± 0.05*	75.4 ± 0.10*	1.10 ± 0.5
	Fe-bLf 20 nM	1.90 ± 0.30	9.30 ± 3.30	87.7 ± 3.40	1.10 ± 0.20
	Fe-bLf 40 nM	3.75 ± 1.95	19.35 ± 6.85	74.00 ± 8.80	2.90 ± 0.00
MCF-7	Untreated	3.40 ± 3.20	5.25 ± 3.25	88.60 ± 7.30	2.70 ± 0.90
	Apo-bLf 20 nM	2.50 ± 0.40	22.85 ± 9.65	64.95 ± 3.85	9.75 ± 6.15
	Apo-bLf 40 nM	6.15 ± 0.55	40.7 ± 7.30*	51.00 ± 6.70*	2.20 ± 0.00
	Fe-bLf 20 nM	12.30 ± 0.30	41.3 ± 1.40*	45.45 ± 1.65*	0.95 ± 0.05
	Fe-bLf 40 nM	21.65 ± 1.65*	43.5 ± 12.10	34.15 ± 10.05*	0.70 ± 0.40

Annexin-V-Fluos staining results determined via Flow cytometry. Total populations acquired were plotted in terms of both single and dual fluorophores. Dual channel plots were divided in to quadrants: Q1: Necrotic cells, Q2: Late apoptotic/Dead cells, Q3: Viable cells, Q4: Early apoptotic cells. Single channel plots were used to determine total numbers of cells labelled with Annexin-V or propidium iodide. Table 1 shows average values for individual gating of both dual and single channel plots + SEM. Statistical analysis was performed via Student’s t-test

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