Fig. 8

BLf reduces survivin expression in breast cancer cell lines. Western blotting for survivin of MDA-MB-231 and MCF-7 cell lysates following treatments for 48 h with Apo and Fe-bLf at 20, 30 and 40 nM. Lysates were collected and 100 μg loaded and run on standard SDS-PAGE followed by transfer to PVDF membranes. Membranes were then blocked with 1% skim milk followed by incubation with anti-survivin primary antibody (Santa Cruz) and anti-mouse secondary antibody (Sigma-Aldrich). Membranes were viewed via XRS camera. Band densities determined by ImageJ software and compared with untreated (0 nM). Separate, identical gels were run for Tubulin which were used to normalize band densities. Band densities determined by ImageJ software and compared with untreated (0 nM). Separate, identical gels were run for Tubulin (Santa Cruz) which were used to normalize band densities. Band density analysis was performed using ImageJ software. Fold change was calculated relative to untreated cells. Relative fold change values per band are indicated below blots as well as plotted on graphs