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Fig. 5 | BMC Cancer

Fig. 5

From: MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism

Fig. 5

MiR-217 directly targets AEG-1 in SW480 cells. (a) Predicted binding site of human miR-217 to the 3’UTR of AEG-1 by TargetScan. (Top panel) The mutation of miR-217 binding site in the 3’UTR of AEG-1. (b) The reporter plasmids containing wild-type or mutant 3’UTR of AEG-1 was co-transfected with miR-217 mimics or mimics NC. The assay was performed twice in triplicate. The relative luciferase activity was obtained by Firefly luciferase activity normalized against Renilla luciferase activity. (c) The effects of overexpression of miR-217 on AEG-1 expression at mRNA level and protein level. (d) The effects of inhibition of miR-217 on AEG-1 expression at mRNA level and protein level. *P < 0.05, **P < 0.01

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BMC Cancer

ISSN: 1471-2407

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