Fig. 2

Efficacy of DT310 and DT320 delivery intraperitoneally (a-d) or via osmotic pump (e-h) in an orthotopic 4T1 syngeneic mouse model of triple-negative breast cancer compared to doxorubicin and docetaxel. An optimized number of 7,500 GFP-labeled cells were injected into the 4th mammary fat pad. Treatment began on day 7 following implantation, which previous studies have shown is when micrometastatic cells begin arriving in the lungs. A and E show the change in volume of the primary tumor in response to treatments. Panels B and F show the effects of treatments on the average number of small lung micrometastases per lung, which are defined as 1–10 cells without vascularization. Panels C and G show the effects of treatment on the average number of large micrometastases per lung, which are defined as clumps of >10 cells without vascularization. Panels D and H show the effects of treatment on the average numbers of lung macrometastases per lung, which are defined as vascularized clumps of cells. Data are means +/−SEM, * = P <0.05, ** = P <0.01 by two-way ANOVA and repeated measures post-test (panels A and E) or by one-way ANOVA with Tukey post-test (panels B-D; F-H)