Table 1 List of primer sequences used for quantitative RT-PCR of the genes indicated
Gene | Forward (5' to 3') | Reverse (5' to 3') |
|---|---|---|
ANAP2 | GACAGGAGTTGTTAGTGGCCT | TCCAGGACCGAGTGTAGCC |
ATM | ACGTTACATGAGCCAGCAAAT | GAAAATGAGGTGGATTAGGAGCA |
ATR | TCTCAGCCAACCTCCGTGAT | GCAGAAGTCTCGTTATGATCCAA |
CCND1 | GTGCTGCGAAGTGGAAACC | ATCCAGGTGGCG ACGATCT |
CCNE1 | GAGCCAGCCTTGGGACAATAA | GCACGTTGAGTTTG GGTAAACC |
CDC34 | GACGAGGGCGATCTATACAACT | GAGTATGGGTAGTCGATGGGG |
CDK2 | GCTAGCAGACTTTGGACTAGCCAG | AGCTCGGTACCACAGGGTCA |
CDK4 | ATGTTGTCCGGCTGATGGA | CACCAGGGTTACCTTGATCTCC |
CDK6 | GCGCCTATGGGAAGGTGTTC | TTGGGGTGCTCGAAGGTCT |
CDKN1A | CGCTAATGGCGGGCTG | CGGTGACAAAGTCGAAGTTCC |
CDKN1B | CTGCAACCGACGATTCTTCTACT | GGGCGTCTGCTCCACAGA |
CUL1 | ACCAGTCAAACCAAGCACGAG | GTCTGCCCCTTTTTCGACTTAG |
CUL2 | ACGACAATAAAAGCCGTGGTC | GGTTCAGGATAGGCCACACATA |
CUL3 | TGTGGAGAACGTCTACAATTTGG | TGCGCCTCTGTCTACGACT |
SKP2 | ACCTCCAGGAGATTCCAGACC | CCCAGGTTTGAGAGCAGTTCC |
TP53 | CCTCAGCATCTTATCCGAGTGG | TGGATGGTGGTACAGTCAGAGC |
NBPF1 | GCGAGGCTGCCCGAGCTTCT | GACTTCGCGTAACTTCCCATTCA |
EGFP | GAGCTGAAGGGCATCGACTT | TCTGCTTGTCGGCCATGAT |