Fig. 5From: NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth inhibitory effects by inducing a G1 cell cycle arrestEGFP-luciferase and EGFP-NBPF1 expression in the cell lines used for flow cytometry in this study. Different cell lines (HEK293T, HEK293T_shRNAp53, SH-SY5Y, NLF and SK-N-AS) were transfected with plasmids expressing either EGFP-luciferase or EGFP-NBPF1 in order to investigate cell cycle distribution. As a control for efficient transfection and expression of EGFP-luciferase or EGFP-NBPF1, we took a fraction of the cells and prepared equal amounts of total cellular lysates for SDS-PAGE. These lysates were immunoblotted with an anti-NBPF antibody (sc-82241) to detect overexpressed NBPF1 protein, with an anti-EGFP antibody to detect the fusion proteins EGFP-luciferase (indicated by *) and EGFP-NBPF1 (indicated by **), or with an anti-actin antibody, which served as a loading control. All cell lines transfected for flow cytometry analysis showed effective expression of EGFP-luciferase or EGFP-NBPF1 proteinsBack to article page