Fig. 10

NBPF1 induces cell death in an NB cell line with wild-type p53 and inhibits cell cycle progression in NB cell lines with mutated p53. Three different NB cell lines were transfected with plasmids encoding either EGFP-luciferase (left panels) or EGFP-NBPF1 (right panels) and cell cycle profiles were analyzed 48 h post-transfection. Transfection efficiency is represented as percentage in the left panels. The different cell cycle phases are shown only for the effectively transfected (EGFP-positive) subpopulations (right panels). Cells in G1 phase (2n DNA content) and cells in G2 phase (4n content) are annotated on the histograms and calculated as percentage of the whole population. Overexpression of NBPF1 in SH-SY5Y cells, bearing a wild-type TP53 gene, does not result in G1 cell cycle arrest, but induces cell death (sub-G1 peak, indicated by arrow) (top panel). Overexpression of NBPF1 in two NB cell lines, NLF (middle panel) and SK-N-AS (bottom panel), with mutated p53 induces G1 cell cycle arrest