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Fig. 3 | BMC Cancer

Fig. 3

From: The role of c-Src in the invasion and metastasis of hepatocellular carcinoma cells induced by association of cell surface GRP78 with activated α2M

Fig. 3

c-Src interacts directly with GRP78 on the cell surface of HCCs. (a) Western blot analysis of the tyrosine-phosphorylated proteins in serum starved QGY-7703 cells treated with vehicle or α2M*. (b) Far western blot analysis of GRP78 binding proteins in the whole cell lysates from serum starved QGY-7703 cells and PLC cells using biotin conjugated rhGRP78 as bait. (c) Co-immunoprecipitation analysis of the interaction between GRP78 and c-Src in the plasma membrane extracts from serum starved QGY-7703 cells using anti-GRP78 (Abcam) as precipitating antibody. Upper, SDS-PAGE; bottom, western blot. (d) GST pulldown analysis of the interaction between GRP78 and c-Src in the plasma membrane extracts from serum starved QGY-7703 cells using GST-GRP78 as the bait. Upper, SDS-PAGE; bottom, western blot

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BMC Cancer

ISSN: 1471-2407

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