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Fig. 4 | BMC Cancer

Fig. 4

From: Mitogen- and stress-activated Kinase 1 mediates Epstein-Barr virus latent membrane protein 1-promoted cell transformation in nasopharyngeal carcinoma through its induction of Fra-1 and c-Jun genes

Fig. 4

Knockdown of MSK1 suppressed LMP1-promoted CNE1 cell proliferation and anchorage-independent growth. (a) The expression of MSK1 was analyzed in the CNE1 cells stably transfected with si-mock or si-MSK1 by Western blotting. si-MSK1 efficiently suppressed the endogenous MSK1 protein level in CNE1 cells. (b) CNE1 cells stably expressing si-mock or si-MSK1 were transfected with pcDNA3.0 or pcDNA3.0-LMP1, and cell proliferation was estimated using CCK-8 assay. Data are presented as mean ± SD for three independent experiments. *P < 0.05; **P < 0.005, compared with si-mock/LMP1 cells. (c) The effect of MSK1 knockdown on LMP1-induced cell cycle distributions was analyzed by flow cytometric assay. Data are expressed as the percentage of cells in G0/G1, S, or G2/M phase. *P < 0.05; **P < 0.005, compared with si-mock/LMP1 cells. (d) CNE1 cells stably expressing si-mock or si-MSK1 were transfected with pcDNA3.0 or pcDNA3.0-LMP1, and soft agar assay was performed to detect the anchorage-independent growth capability. *P < 0.005, compared with si-mock/LMP1 cells

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BMC Cancer

ISSN: 1471-2407

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