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Fig. 1 | BMC Cancer

Fig. 1

From: Mitochondrially targeted vitamin E succinate efficiently kills breast tumour-initiating cells in a complex II-dependent manner

Fig. 1

NeuTL and MCF7 spheres are a plausible model of TICs. Neu TL cells were cultured in serum-containing and sphere medium (A) and assessed for selected stemness genes by qPCR (C). MCF7 cells were cultured in adherent and ‘sphere’ medium (B) and assessed for selected stemness genes by qPCR (D). (E) NeuTL adherent and sphere cells were grafted s.c. in FVB/N c-neu mice (106 cells per animal) and tumour volume assessed using USI. The images on the right are representative USI scans of tumours taken on the given days (indicated by arrows in the graph on the left). (F) Sections of tumours were stained by H&E for morphology, also showing regions of low and more differentiated cancer cells. (G) Tumour sections were evaluated for the level of erbB2 using WB and IHC. In all cases, the level of stemness genes in sphere cells was related to that in their adherent counterparts, set as 1. Data are mean values ± S.D. (n = 3). The symbol ‘*’ indicates statistically significant differences in the level of mRNA in adherent and sphere cells with p < 0.05. Images in panels A, B, E, F and G are representative of three independent experiments

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