Figure 5
From: Enhancement of hypoxia-activated prodrug TH-302 anti-tumor activity by Chk1 inhibition
ADZ7762 enhanced TH-302-mediated cytotoxicity only in HDR-proficient but not in HDR-deficient cells regardless of NHEJ status. HDR-proficient CHO AA8 (A), HDR-deficient CHO irs1SF (B), HDR-deficient CHO UV41 (C), NHEJ-proficient CHO K1 (D) and NHEJ-deficient CHO xrs5 cells (E) were seeded 24 h prior to experiment. After compound addition, cells were incubated for 2 h with TH-302 and 0.1 μM AZD7762 under either normoxia (21% O2) or hypoxia (N2). After wash, cells were continuously incubated for additional 70 h in complete medium containing 0.1 μM of AZD7762. The viable cells were quantified using AlamarBlue and IC50 values were calculated using Prism software. The data are the representative of three independent experiments.