Figure 4
From: Enhancement of hypoxia-activated prodrug TH-302 anti-tumor activity by Chk1 inhibition
Enhanced γH2AX signal by co-treatment of TH-302 and AZD7762. HT29 cells were treated with TH-302 alone at the indicated concentrations, 0.1 μM AZD7762 alone, or combined treatment of TH-302 and 0.1 μM AZD7762 under either air (21% O2) or hypoxia (N2) for 2 h and then washed. The signal of γH2AX was detected by immunofluorescence microscopy (A and B) after additional 4 h incubation in the presence of 0.1 μM AZD7762 for combination group and AZD7762 group. The blue-fluorescent nucleic acid stain DAPI was used as a nuclear counterstain. Immunoblot (C) data were generated after 22 h additional incubation in the presence of 0.1 μM AZD7762 for combination group and AZD7762 group. The data are the representative of two independent experiments.