In addition to the homogeneous pattern in all three specimens, the serum in A from a patient with DCIS had a mixed pattern with the lower solid arrow depicting anti-centrosome antibodies while an upper solid arrow shows MNDs. B corresponds to a serum from a patient with IDC. A striped arrow in A and B show a positive metaphase plate as seen in the homogeneous pattern. The arrow in C points to mitochondrial fluorescence in a specimen from a healthy woman with BBD. The immunoblots done at a 1:500 dilution in all figures, showed the presence of many more IgG antibodies than those recognized by IFA.