Optimisation of operating parameters for determination of MCT4 by immunofluorescence (IF) in control cell lines spiked into volunteer blood. The figure again contains a gallery of images obtained by the CellSearch system in 4 different channels from a total of 9 different cells. The images were generated at the optimum integration time of 0.8 seconds for detection of MCT4. Identification of the 4 single channels and the composite are as defined in Figure 4. MCT4 was only detected in the 4th channel in the positive control cell line MDAMB231 (see Figure 1) that had been stained with MCT4 antibody, but was absent in unstained MDAMB231 cells and the negative control cell (see Figure 1) K562 stained for MCT4.