URG4/URGCP is upregulated in HCC cell lines. A. Western blotting analysis of URG4/URGCP protein expression in two normal liver cell lines and seven HCC cell lines; α-Tubulin was used as a loading control. Lower panel, quantification of Western blotting data relative to Lo2 cells. B. Real-time PCR quantification of URG4/URGCP mRNA expression in two normal liver cell lines and seven HCC cell lines. Transcript levels were normalized to GAPDH and expressed relative to Lo2 cells. Data is mean ± SD of three independent experiments; ** P < 0.01.