Figure 5

In vivo T-cell activation and suppression of macrophages mediated by PLX3397 and PLX4032. Gene expression signature overlap maps were used to compare the similarity of drug-treated full tumor gene expression profiles with a panel of T-cell and monocyte (Mono) gene signatures from a reference immune cell signature database (the Rank-Rank Hypergeometric Overlap (RRHO) algorithm and the Differentiation Map (DMAP) database), a) PLX3397 treatment vs vehicle control (VC); b) PLX4032 vs vehicle; c) combined drug treatment (Combo) vs vehicle. d) Gene expression heat map for macrophage signature genes in F4/80(+) CD11b(+) macrophages FACS-sorted from SM1 tumors treated with PLX3397 or PLX4032 for 5 days. e) Gene expression heat map for T-cell signature genes in CD3(+) CD8(+) T-cells from SM1 tumors. Color scale, log₂-transformed fold change expression (red, high; green, low) for each gene (row) normalized to the value for the vehicle control-treated tumors.