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Figure 4 | BMC Cancer

Figure 4

From: Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro

Figure 4

Analysis of ADCC in control cell lines. Effects of sEpCAM on catumaxomab-dependent cell mediated cytotoxicity (A) HEK EpCAM-YFP, and HEK EpICD-YFP cells were incubated without or with 1 ng/mL catumaxomab together with a 10-fold excess of human PBMNCs. PBMNCs with catumaxomab were efficiently killing EpCAM-YFP cells, but did not attack EpICD-YFP cells. Living cells are displayed by fluorescence microscopy (left picture). Incubation with 1 ng/mL EpEX already inhibited catumaxomab -dependent cell mediated cytotoxicity on EpCAM-YFP overexpressing HEK cells. Bars indicate 100 μm. (B) HEK EpCAM-YFP, and HEK EpICD-YFP cells were incubated without or with 1 ng/mL catumaxomab together with a 10-fold excess of human PBMNCs and increasing concentrations of EpEX (0, 0.2, 1, 5 ng/mL). YFP positive events were counted by flow cytometry (488 nm) and normalized to control cells without catumaxomab/PBMNC incubation (100%). ADCC was repeated three times (Mean ± SEM).

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