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Figure 1 | BMC Cancer

Figure 1

From: Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro

Figure 1

EpCAM standard and stable control cells lines for ELISA. (A) Coomassie brilliant Blue staining of PAGE gel in the presence of two different standard proteins (GRP78 and BAP). Purity of the EpEX was approx. 93% as determined by Page Blue staining and densitrometric analysis. Molecular weight marker indicates reference proteins in KDa. EpEX-FLAG (293 aa) has an estimated size of 35 KDa. (B) Real-Time confocal microscopy pictures of living HEK293FT cell lines expressing different EpCAM isoforms fused to YFP: EpCAM-YFP shows expression only on cell membrane, EpEX-TM-YFP is detectable in the endoplasmatic reticulum and on cell membrane. Due to the lack of transmembrane region EpICD-YFP is found in cytosol and nucleus of living cells.

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BMC Cancer

ISSN: 1471-2407

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