Figure 1

Mitochondrial respiratory modifiers (MRMs; PS3, DNP & MB) induces glycolysis. Glucose consumption and lactate production observed every hour till 4 hours of the drug treatment is presented as average per hour in BMG-1 (A), OCT-1 (B) and HEK293 (C) cells. (D) Protein expression profile of glucose transporter, glycolytic enzymes and transcriptional regulator of glycolysis HIF1α is shown in BMG-1 cells. The data shows western blots and their derived quantitative values from the densitometry. (E) Relative hexokinase enzymatic activity in un-irradiated and irradiated (5 Gy γ-rays) BMG-1 cells is presented as absorbance at 340 nm obtained from coupled enzymatic assay. The concentration of different treatments used was as follows, PS3, 25 μg/ml; DNP, 1 μM; MB, 25 μM. The data shown are the mean values (±1 SD) of nine observations from three independent experiments. Statistical significance *p < 0.05.