Expression of SAM68 mRNA and protein in paired muscle invasive bladder cancer tissues and adjacent non-carcinoma tissues. (A) Quantitative PCR showed the mRNA level of SAM68 was much higher in bladder cancer tissues compared with adjacent non-carcinoma tissues. Expression levels were normalized for GAPDH. Error bars represent the standard deviation (SD) calculated from three parallel experiments. (B) Immunohistochemistry analysis was performed to detect the SAM68 protein level in tumor (T) and non-carcinoma tissues (N) from the same patient.