Fig. 4

miR-34a antagonizes Smad4-mediated TGF-β induction of EMT in vitro.a Both of the QBC939 and HuCCT1 cells were transfected with miR-34a mimics or scramble oligos, and treated with or without TGF-β simultaneously. After 48 hrs, cells lysate were examined with indicated antibodies by Western blot. β-actin was used as loading control. b Morphological investigations of the EHCC cells. Both QBC939 and HuCCT1 cells were transfected with miR-34a mimics then treated with or without 5 ng/ml of TGF-β for 24 hrs. Cells transfected with miR-34a scramble oligos were used as the controls (original magnification: ×200)