Figure 2
Effect of FGF9 treatment on intracellular signaling in gastric cancer cells. (A) Phosphorylation of Akt and ERK in gastric cancer cells treated with FGF9. AGS (4 × 105) and MKN28 (4 × 105) were cultured in six-well plates and treated with various concentrations of FGF9 for 30 min. Extracted protein was analyzed by Western blotting, as described in Materials and Methods. (B) Time course change in Akt and ERK phosphorylation in gastric cancer cells treated with FGF9. AGS and MKN28 cells were similarly treated with FGF9 (10 ng/ml) for the indicated times. (C) Effect of anti-FGF9 neutralizing antibody on FGF9-induced Akt and ERK phosphorylation in gastric cancer cells. AGS and MKN28 cells were pretreated with anti-FGF9 antibody (Ab; 1 μg/ml) for 45 min and then stimulated with FGF9 (10 ng/ml) for 30 min.