Figure 5

Mitochondrial ND6 gene nonsense and missense mutation over-produces the ROS. ROS level was analyzed by flow cytometry. (A) Representative flow cytometry graphs of A549 cells with the wild type mND6 (normal), cells with missense mutation and nonsense mutations of mitND6 gene and cells treated with rotenone. Histogram represented the ROS level in cells. (B) Histogram showed the relative ROS level of cells. Data represent mean ± SEM from 4 independent experiments; **, P < 0.01.