Table 2 Intra- and Inter-day precision (% RSD) and accuracy (% Bias) of the HPLC-UV method for determination of 5-FU in rat plasma and lymphatic fluid (5Â days, 5 replicates per day)
| Â | Â | Intra-day | Â | Â | Inter-day | Â | Â |
|---|---|---|---|---|---|---|---|
Matrices | Nominal concentration (μg/mL) | Observed concentration (ng/mL) | Precision (% RSD) | Accuracy (% Bias) | Observed concentration (ng/mL) | Precision (% RSD) | Accuracy (% Bias) |
Plasma | 0.5 | 0.47 ± 0.09 | 18.90 | −5.55 | 0.48 ± 0.08 | 16.58 | −4.30 |
|  | 1 | 1.02 ± 0.08 | 7.91 | 2.25 | 1.02 ± 0.09 | 8.81 | 1.57 |
|  | 5 | 4.87 ± 0.24 | 4.91 | −2.58 | 4.79 ± 0.38 | 8.00 | −4.80 |
|  | 10 | 10.15 ± 0.28 | 2.75 | 1.52 | 10.10 ± 0.22 | 2.13 | 0.99 |
|  | 50 | 49.98 ± 0.04 | 0.08 | −0.04 | 50.29 ± 0.66 | 1.30 | 0.58 |
Lymphatic | 0.5 | 0.54 ± 0.03 | 8.67 | 7.60 | 0.51 ± 0.03 | 5.72 | 1.98 |
fluid | 1 | 1.06 ± 0.06 | 5.24 | 6.30 | 1.03 ± 0.05 | 4.63 | 3.06 |
|  | 5 | 4.86 ± 0.19 | 3.96 | −2.74 | 4.98 ± 0.11 | 2.24 | −0.45 |
|  | 10 | 10.03 ± 0.13 | 1.31 | 0.30 | 10.03 ± 0.13 | 1.27 | 0.28 |
|  | 50 | 50.01 ± 0.01 | 0.02 | 0.01 | 49.91 ± 0.23 | 0.46 | −0.17 |