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Table 2 Intra- and Inter-day precision (% RSD) and accuracy (% Bias) of the HPLC-UV method for determination of 5-FU in rat plasma and lymphatic fluid (5 days, 5 replicates per day)

From: Local pelvic irradiation modulates Pharmacokinetics of 5-Fluorouracil in the plasma but not in the Lymphatic System

   Intra-day    Inter-day   
Matrices Nominal concentration (μg/mL) Observed concentration (ng/mL) Precision (% RSD) Accuracy (% Bias) Observed concentration (ng/mL) Precision (% RSD) Accuracy (% Bias)
Plasma 0.5 0.47 ± 0.09 18.90 −5.55 0.48 ± 0.08 16.58 −4.30
  1 1.02 ± 0.08 7.91 2.25 1.02 ± 0.09 8.81 1.57
  5 4.87 ± 0.24 4.91 −2.58 4.79 ± 0.38 8.00 −4.80
  10 10.15 ± 0.28 2.75 1.52 10.10 ± 0.22 2.13 0.99
  50 49.98 ± 0.04 0.08 −0.04 50.29 ± 0.66 1.30 0.58
Lymphatic 0.5 0.54 ± 0.03 8.67 7.60 0.51 ± 0.03 5.72 1.98
fluid 1 1.06 ± 0.06 5.24 6.30 1.03 ± 0.05 4.63 3.06
  5 4.86 ± 0.19 3.96 −2.74 4.98 ± 0.11 2.24 −0.45
  10 10.03 ± 0.13 1.31 0.30 10.03 ± 0.13 1.27 0.28
  50 50.01 ± 0.01 0.02 0.01 49.91 ± 0.23 0.46 −0.17
  1. Data expressed as mean ± SD.