Table 3 Quantitative PCR primers for determination of mRNA expression levels of polymerase\( \boldsymbol{\upalpha} \), \( \boldsymbol{\updelta} \), \( \boldsymbol{\upvarepsilon} \), AFP and\( \boldsymbol{\upbeta} \)-actin
From: DNA Polymerases as targets for gene therapy of hepatocellular carcinoma
gene detected | gene code | primer sequence | product size(bp) |
|---|---|---|---|
polymerase α | NM_016937.3 | F: 5- TTCTGACAAGTCCCTGTACACC −3 | 240 |
R: 5- TAGGATTTCACGGCACAACCA −3 | |||
polymerase δ | NM_002691.3 | F: 5- CCCTACGTGATCATCAGTGC −3 | 107 |
R: 5- AGGTAGTACTGCGTGTCAATGG −3 | |||
polymerase ε | NM_006231.2 | F: 5- CCTACGACTCCTCTGCCATC −3 | 219 |
R: 5- CGTAGTGCTGGGCAATGTTC −3 | |||
AFP | NM_001134.1 | F: 5- ACCTCGTCGGAGCTGATGG −3 | 204 |
R: 5- TGGCCTCCTGTTGGCATATG −3 | |||
β-actin | NM_001101.3 | R: 5- CCGATCCACACGGAGTACTT −3 | 406 |