Table 2 PCR primers and reaction conditions for identification of recombinant adenoviruses
From: DNA Polymerases as targets for gene therapy of hepatocellular carcinoma
gene detected | primer sequence | PCR reaction condition | product size(bp) |
|---|---|---|---|
miR-polα-δ-ε | F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3 | 95°C , 5 min; 94°C , 30s, 50°C , 30s, 72°C , 45 s, 35 cycles; 72°C , 5 min | 142, 284, 426 |
R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3 | |||
polyA | F: 5-AGATCTTGTGCCTTCTAGTTGCCAGC-3 | 95°C , 5 min; 94°C , 30s, 53°C , 30s, 72°C , 30s, 35 cycles; 72°C , 5 min | 237 |
R: 5-GGATCCGCCATAGAGCCCACCGCATC-3 | |||
AFP promoter | F: 5-AGATCTCAGATTGAATTATTTGC-3 | 95°C , 5 min; 94°C , 30s, 55°C , 30s, 72°C , 90s, 35 cycles; 72°C , 5 min | 1018 |
R: 5-GGATCCAAATCATGCTGAAATT-3 | |||
recombinant caspase3 | F: 5-AGATCTGGCTAACTAGAGAACCCA-3 | 95°C , 5 min; 94°C , 30s, 58°C , 30s, 72°C , 60s, 35 cycles; 72°C , 5 min | 610 |
R: 5-GGATCCCCCATCAACTTCATCGTGATAAAAATAGAGTTC-3 |