Table 1 PCR primers for amplification of artificial microRNAs
From: DNA Polymerases as targets for gene therapy of hepatocellular carcinoma
artificial microRNA | Â | primer sequence | product size(bp) |
|---|---|---|---|
miR-polα | 1st round | F: 5-TGCTGTTGACAGTGAGCGCGCAGATCATGTGTGAGCTAAATAGTGAAGCCACAGATGTA-3 | 97 |
R: 5-TCCGAGGCAGTAGGCATGCAGATCATGTGTGAGCTAAATACATCTGTGGCTTCACTATT-3 | |||
2nd round | F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3 | 142 | |
R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3 | |||
miR-polδ | 1st round | F: 5-TGCTGTTGACAGTGAGCGACGGGACCAGGGAGAATTAATATAGTGAAGCCACAGATGTA-3 | 97 |
R: 5-TCCGAGGCAGTAGGCAGCGGGACCAGGGAGAATTAATATACATCTGTGGCTTCACTATA-3 | |||
2nd round | F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3 | 142 | |
R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3 | |||
miR-polε | 1st round | F: 5-TGCTGTTGACAGTGAGCGAGGTTCCCACTTGCTGCTCAATTAGTGAAGCCACAGATGTA-3 | 97 |
R: 5-TCCGAGGCAGTAGGCACGGTTCCCACTTGCTGCTCAATTACATCTGTGGCTTCACTAAT-3 | |||
2nd round | F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3 | 142 | |
R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3 |