Figure 1
From: DNA Polymerases as targets for gene therapy of hepatocellular carcinoma
Illustration of hairpin structure of artificial microRNAs and recombinant adenoviruses. A: DNA sequences of artificial miRNAs targeting DNA polymerases α, δ and ε were based on natural structure of human miR-30. The bolds were core sequences acquired from RNAi Codex. B: a) Recombinant adenovirus Ad/AFP-Casp-AFP-amiR containing two expression cassettes to express artificial microRNAs targeting DNA polymerase α, δ, ε and active recombinant Caspase3. b) Recombinant adenovirus Ad/AFP only containing AFP promoter used as control.