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Table 2 Patterns of the in situ distribution of PGs in HNSCC lesions (% of cases)

From: Proteoglycan-based diversification of disease outcome in head and neck cancer patients identifies NG2/CSPG4 and syndecan-2 as unique relapse and overall survival predicting factors

PG Tumor cell positivity1 Overall staining intensity2 Subcellular localization3 Stromal expression
GPC1 - (27.5) - (27.6) Membrane - - (92.0)
  + (29.2) + (33.1) Cytoplasmic - + (6.7)
  ++ (33.1) ++ (30.1) Both (72.4) ++ (0.6)
  +++ (7.4) +++ (6.7)    +++ (0.6)
  ++++ (2.5) ++++ (2.5)    ++++ -
GPC3 - (81.6) - (81.6) Membrane - - (100.0)
  + (14.1) + (14.1) Cytoplasmic - + -
  ++ (3.1) ++ (4.3) Both (18.4) ++ -
  +++ (1.2) +++ -    +++ -
  ++++ - ++++ -    ++++ -
GPC4 - (58.9) - (59.5) Membrane - - (81.0)
  + (20.9) + (14.1) Cytoplasmic (40.5) + (5.5)
  ++ (19.0) ++ (25.8) Both - ++ (12.9)
  +++ (1.2) +++ (0.6)    +++ (0.6)
  ++++ - ++++ -    ++++ -
GPC6 - (62.2) - (62.6) Membrane - - (90.2)
  + (30.1) + (31.9) Cytoplasmic (37.4) + (9.8)
  ++ (7.4) ++ (5.5) Both - ++ -
  +++ - +++ -    +++ -
  ++++ - ++++ -    ++++ -
SDC1 - (8.6) - (8.6) Membrane (42.9) - (79.1)
  + (31.3) + (25.8) Cytoplasmic (37.4) + (9.2)
  ++ (33.7) ++ (50.3) Both (10.4) ++ (10.4)
  +++ (22.1) +++ (10.4)    +++ (1.2)
  ++++ (4.3) ++++ (4.9)    ++++ -
SDC2 - (88.3) - (88.3) Membrane (1.8) - (26.4)
  + (6.7) + (8.0) Cytoplasmic (8.6) + (20.2)
  ++ (4.3) ++ (3.1) Both - ++ (25.8)
  +++ (0.6) +++ (0.6)    +++ (21.5)
  ++++ - ++++ -    ++++ (6.1)
SDC3 - (65.0) - (65.0) Membrane - - (100.0)
  + (27.0) + (28.2) Cytoplasmic - + -
  ++ (6.1) ++ (6.7) Both (34.4) ++ -
  +++ (1.8) +++ -    +++ -
  ++++ - ++++ -    ++++ -
SDC4 - (80.4) - (80.4) Membrane - - (100.0)
  + (16.0) + (19.6) Cytoplasmic - + -
  ++ (3.7) ++ - Both (17.8) ++ -
  +++ - +++ -    +++ -
  ++++ - ++++ -    ++++ -
NG2/CSPG4 - (34.2) - (34.2) Membrane (62.6) - (96.7)
  + (38.8) + (36.8) Cytoplasmic - + (3.3)
  ++ (21.7) ++ (19.7) Both - ++ -
  +++ (2.6) +++ (7.2)    +++ -
  ++++ (2.6) ++++ (2.0)    ++++ -
  1. 1PG expression was assessed semi-quantitatively according to the arbitrary scoring: “-”, no positively staining cells were detected; “+”, <10% of cells were positive; “++”, ≥10% and <50% of positive cells; “+++”, ≥50% and <90% of positive cells; “++++”, ≥90% of positive cells;
  2. 2Refers to the average staining intensity within the examined lesion, according to the arbitrary scoring: “-” = absent; “+”, faint; “++”, weak; “+++”, moderate; “++++”, strong;
  3. 3Immunostaining was prevalently cell membrane-associated (“Membrane”) or diffuse cytoplasmatic (“Cytoplasmic”).